Device

Part:BBa_K2518010

Designed by: Muhammad Hassan Raza   Group: iGEM17_Peshawar   (2017-10-27)


RFP 2

Our team has successfully validated a newly designed RFP coding device (RFP2) that contains double RFP reporting system. Our designing and wet lab experiments not only proved that our part has properly and efficiently worked but also helped us improve one of the most used parts in the iGEM registry, BBa_J04450.

Results are vivid; our validated part that we named RFP2 is showing brighter fluorescence under UV light and giving more intense color (purplish-red) under visible light than the random RFP device.

             <img class="img-responsive" src="T--Peshawar--Improve-1.png" width="500"></img>

RFP2 under UV light along with controls, as brighter and beautiful as neon!

             <img class="img-responsive" src="T--Peshawar--Results-Last-1.jpg" width="500"></img>

RFP2 under visible light, shocking pink and yummy!

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
    Illegal AgeI site found at 1858
    Illegal AgeI site found at 1970
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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